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CARIES-RELATED MICROBIOLOGICAL SCREENING IN CHILDREN UNDER THREE YEARS OF AGE

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Abstract

3¼¼ ÀÌÇÏÀÇ ¾î¸°ÀÌ¿¡¼­ ±¸°­ ³» ¹Ì»ý¹°°ú Ÿ¾×¿ÏÃæ´ÉÀÌ Ä¡¾Æ¿ì½ÄÁõ°úÀÇ °ü·Ã¼ºÀ» ¾Ë¾Æº¸¾Ò´Ù. 87¸íÀÇ ¾î¸°À̸¦ ½ÇÇè´ë»óÀ¸·Î ÇÏ¿© Ä¡ÅÂ¿Í ÀڱؼºÅ¸¾×À» °¢°¢ ¸éºÀ°ú ¸é±¸¸¦ ÀÌ¿ëÇÏ¿© äÃëÇÏ¿´´Ù. 0.94%ÀÇ lactic acid¸¦ Ÿ¾× Ç¥º»¿¡ ÷°¡Çϱâ Àü°ú ÈÄÀÇ pH¸¦ °¢°¢ ÃøÁ¤ÇÏ¿´°í Ÿ¾×Ç¥º»À» ¼øÂ÷ÀûÀ¸·Î Èñ¼®ÇÏ¿© ¼±ÅùèÁö¿Í ºñ¼±ÅùèÁö¿¡ Á¢Á¾ÇÏ¿´´Ù. Mutans streptococci(MS), lactobacilli(LB), total viable count(TVC)ÀÇ Áý¶ô¼ö¿Í Ÿ¾×ÀÇ pH¿Í ¿ÏÃæ´ÉÀ» ¾î¸°ÀÌÀÇ Ä¡¾Æ¿ì½Ä°ú ºñ±³ÇÏ¿© ´ÙÀ½°ú °°Àº °á°ú¸¦ ¾ò¾ú´Ù.
1. ÀڱؼºÅ¸¾×°ú Ä¡Å ¸ðµÎ¿¡¼­ MS¿Í LB°¡ Ä¡¾Æ¿ì½ÄÁõ°ú ³ôÀº Åë°èÇÐÀû À¯ÀǼºÀ» º¸¿´´Ù.
2. Ä¡¾Æ¿ì½ÄÀÇ ¹ß»ýÀ» ¿¹ÃøÇÒ ¼ö ÀÖ´Â ¹Ì»ý¹°ÀÇ Áý¶ô¼ö´Â ´ÙÀ½°ú °°¾Ò´Ù.
1) ÀڱؼºÅ¸¾× 1ml´ç MS Áý¶ô¼ö°¡ 10^(5) ÀÌ»ó
2) Ä¡Å 1ml´ç MS Áý¶ô¼ö°¡ 2¡¿10^(5)ÀÌ»ó
3) ÀڱؼºÅ¸¾× 1ml´ç LB Áý¶ô¼ö°¡ 10^(3) ÀÌ»ó
4) Ä¡Å 1ml´ç LB Áý¶ô¼ö°¡ 10^(3) ÀÌ»ó
3. Ÿ¾×ÀÇ pH¿Í ¿ÏÃæ´ÉÀº À¯¾Æ±â¿ì½ÄÁõ°ú °ü·ÃÀÌ ¾ø¾ú´Ù.
4. MS °Ë»ç´Â LB °Ë»ç º¸´Ù ³ôÀº ¿¹ÃøÄ¡(predictive value)¿Í ±³Â÷ºñ(odds ratio)¸¦ º¸¿´´Ù.
5. MS Áý¶ô¼ö´Â ¾î¸°ÀÌ¿¡ À־ Ä¡¾Æ¿ì½Ä ¹ß»ýÀ» ¿¹ÃøÇÒ ¼ö ÀÖ´Â °¡Àå ¹ÏÀ»¸¸ÇÑ ¹Ì»ý¹°ÇÐÀû °Ë»ç·Î ÀϹÝÀûÀÎ ¼¼±Õ¹è¾ç¹ýÀ» ÀÌ¿ëÇϰųª »óÇ°È­µÈ °Ë»ç±â±¸¸¦ ÀÌ¿ëÇÏ¿© ½±°Ô ÀÓ»ó¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù.
To evaluate microbial data and salivary measurements from clinically compatible, culture-based screen¡þing procedures employed with children younger than 36 months old. Plaque and stimulated saliva speci¡þmens were collected from 87 children. The pH of each saliva sample was measured before and after 0.94% lactic acid was added. Specimens were diluted and plated on selective media and non-selective media. Data collected were counts of mutans streptococci (MS) and lactobacilli (LB). In addition, total viable counts (TVC) of specimens, salivary pH and buffering capacity were also assessed. Each variable was compared to caries status of subjects.
According to this study, the results were as followed:
1. Highly significant correlation with caries rates were found for counts of MS and LB.
2. The specific counts/ml saliva or plaque above which caries is predicted, or below which caries is not predicted were as follows:
1) Saliva MS ; 105
2) Plaque MS, 2 x 105
3) Saliva LB; 103
4) Plaque LB; 103 3. Salivary pH and buffering capacity versus caries status were not significant.
4. Microbial screening methods based on mutans streptococci had higher predictive values and odds ra¡þtios than methods for lactobacilli.
5. MS counts were clearly the best indicators of caries status in young children. This measurement can easily be obtained in a dental clinical setting both by conventional culture techniques, or commercial kits for MS recovery.

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Ÿ¾×¿ÏÃæ´É;Ä¡ÅÂ;ÀڱؼºÅ¸¾×;mutans streptococci;lactobacilli;Plaque;Stimulated saliva;Mutans streptococi;Lactobacilli;Salivary pH;Buffering capacity

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